The National Videogame Arcade (NVA) is to re-locate from its current base in Nottingham to Sheffield.
The games centre and museum, which opened in 2015, said it had become “increasingly hard to work” in the “lovely but eccentric” listed Hockley building in which it is based.
Games Workshop co-founder Ian Livingstone issued a call for the games industry to support the centre in 2016.
The Carton Street site will close after a summer programme of events.
The NVA will close in September 2018 and reopen in Sheffield in October, say the British Games Institute (BGI), which merged with the NVF and will also be moving to Sheffield.
The NVA will be based at the Kollider building in the Castlegate area of the city.
An education and research facility will remain in Nottingham, and GameCity events will also return.
A spokesman for Nottingham City Council said it is “sorry” to see the NVA move but expects to find new tenants to occupy the site “quickly”.
“While the NVA’s move is disappointing, Nottingham remains a nationally significant tech centre,” they said.